Disseminated purulent peritonitis outcome affects NKT cell phenotype

The aim of our study was to investigate the main characteristics peripheral blood NKT cell phenotype in patients with disseminated purulent peritonitis (DPP) dynamics postoperative period, depending on disease outcome. Fifty-two acute surgical diseases and injuries abdominal organs complicated by DPP, 68 healthy individuals control group, were examined. Blood sampling performed before surgery (preoperative period), as well day 7, 14 21 period. All DPP divided into two groups outcome period: favorable (n = 34); unfavorable 18). Study lymphocytes flow cytometry using direct immunofluorescence whole samples monoclonal antibodies. low relative absolute level cells observed regardless preoperative At same time, returned normal only after surgery. Patients end examination period had normalized quantity mature NKT-lymphocytes significantly decreased cytotoxic which apparently associated migration such subsets site inflammation. A reduced non-classical (expressing CD8 marker) cytokine-producing detected these treatment. high CD11b+ already while increased proportion expressing activation markers (CD28 CD57) that immediately outcome, it recovered closer examination. defined features characterize disturbances subset ratio mechanisms functioning this fraction. This determines a need develop immunotherapeutic methods aimed at stimulating immunoregulatory activity cells.